Table 1: Agonistic potency of physiologically active substances to induce signals of hMRGPRD.

EC50 (μM)GTPγSCalcium fluxIP

βAla 2 1 9 . 8 ± 1 1 5 . 7 2 2 . 6 ± 1 1 . 4 3 5 3 . 3 ± 2 1 7 . 0
βAIBA 8 2 1 . 7 ± 1 2 0 . 9 5 3 . 4 ± 2 7 . 3 3 7 8 . 3 ± 2 9 0 . 5
GABANot determined 4 3 8 5 ± 8 7 1 . 1 >1000
DES 5 0 . 7 ± 1 8 . 1 1 0 . 4 ± 3 . 0 not available

EC50 (sample concentration producing 50% of the maximal response) was calculated from dose-response curves and shown in μM. Multiple assays were executed to calculate EC50s: calcium flux assay (Calcium flux), GTPγS binding assay (GTPγS), and IP assay (IP).