Identification of Physiologically Active Substances as Novel Ligands for MRGPRD

Table 1

Agonistic potency of physiologically active substances to induce signals of hMRGPRD.

EC50 (μM)	GTPγS	Calcium flux	IP
βAla	219.8±115.7	22.6±11.4	353.3±217.0
βAIBA	821.7±120.9	53.4±27.3	378.3±290.5
GABA	Not determined	4385±871.1	>1000
DES	50.7±18.1	10.4±3.0	not available

EC₅₀ (sample concentration producing 50% of the maximal response) was calculated from dose-response curves and shown in μM. Multiple assays were executed to calculate EC₅₀s: calcium flux assay (Calcium flux), GTPγS binding assay (GTPγS), and IP assay (IP).