Table 2: Antagonistic activity of MU-6840 for hMRGPRD.

Calcium fluxGTPγSIPSpheroid

IC50 (nM) 6 9 1 . 7 ± 1 2 0 . 9 1 7 0 3 . 3 ± 3 5 3 . 9 4 7 3 . 8 ± 1 6 0 . 3 6 5 5 . 9 ± 2 0 4 . 3

IC50 (sample concentration producing 50% of the maximal inhibition) was calculated from dose-response curves and shown in nM. Multiple assays were used to calculate IC50s: calcium flux assay (Calcium flux), GTPγS binding assay (GTPγS), IP assay (IP), and spheroids growth assay (Spheroid).