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Figure 2: rMSC characterization. (a–d) rMSCs, at P80, were characterized by flow cytometry analysis for the expression of the following markers (pink histograms): CD29 (a), CD90 (b), CD34 (c), and CD45 (d). Isotype-matching IgGs were used to determine nonspecific signals (white histograms). (e and f) rMSC differentiation, at P80, after treatment with specific induction media: osteogenic differentiation was evaluated by alizarin red staining that visualizes calcium deposits (e), adipogenic differentiation was evaluated by oil red O staining that labels lipid droplets, not observed in adipogenic treated rMSC cultures at P80 (f). Bar: 100 μm.