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Figure 5: Spontaneous expression of neural and mesengenic markers by undifferentiated rMSCs (P4) after 14 days of culture. Phalloidin staining labeled actin filaments in red (a, d, g, j, m, p, and s), and neural and mesengenic markers were labelled in green (b, e, h, k, n, q, t, and w). Propidium iodide labeled nuclei in red (v), used instead of phalloidin for a better labelling visualization after superimposition, being OPN expressed as dots. Most of cells were, respectively, positive for nestin (b) and III tubulin (e). Numerous cells were NeuN positive with nuclear localization (h). Few cells were, respectively, positive for GFAP (n) and OPN (w). Very few cells were, respectively, positive for NF (k), S100 (q), and PPAR (t). Merges (c, f, i, l, o, r, u, and x). Bars: 50 μm.