Figure 7: rMSC expression of neural markers by immunoblotting. Protein extracts (15 μg), from rMSC or DRG cultures, were separated by 7.5% SDS-PAGE (a) or 13% SDS-PAGE (b) and transferred to a nitrocellulose membrane. Membranes were, respectively, blotted with anti-nestin, anti-III-tubulin, anti-GFAP, and anti-NeuN antibodies. Two bands corresponding to 200 and 220 kDa molecular weight were evident for nestin (a), a 50 kDa band for III tubulin and GFAP (b) immunoblottings. The NeuN blot shows two major bands at 45–50 kDa and additional reactive bands at 66 kDa and between 70 and 90 kDa (b). Actin was used as loading control (a and b).