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Journal of Biomedicine and Biotechnology
Volume 2012 (2012), Article ID 820821, 13 pages
http://dx.doi.org/10.1155/2012/820821
Research Article

Expression of Neural Markers by Undifferentiated Rat Mesenchymal Stem Cells

Dipartimento di Neuroscienze e Tecnologie Biomediche, Facoltà di Medicina e Chirurgia, Università degli Studi di Milano-Bicocca, Via Cadore 48, 20052 Monza, Italy

Received 14 March 2012; Revised 13 September 2012; Accepted 13 September 2012

Academic Editor: Michael D. Coleman

Copyright © 2012 Dana Foudah et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. G. Brooke, M. Cook, C. Blair et al., “Therapeutic applications of mesenchymal stromal cells,” Seminars in Cell and Developmental Biology, vol. 18, no. 6, pp. 846–858, 2007. View at Publisher · View at Google Scholar · View at Scopus
  2. Y. L. Si, Y. L. Zhao, H. J. Hao, X. B. Fu, and W. D. Han, “MSCs: biological characteristics, clinical applications and their outstanding concerns,” Ageing Research Reviews, vol. 10, no. 1, pp. 93–103, 2011. View at Publisher · View at Google Scholar · View at Scopus
  3. O. Bugos, M. Bhide, and N. Zilka, “Beyond the rat models of human neurodegenerative disorders,” Cellular and Molecular Neurobiology, vol. 29, no. 6-7, pp. 859–869, 2009. View at Publisher · View at Google Scholar · View at Scopus
  4. B. Zavan, C. Giorgi, G. P. Bagnara, V. Vindigni, G. Abatangelo, and R. Cortivo, “Osteogenic and chondrogenic differentiation: comparison of human and rat bone marrow mesenchymal stem cells cultured into polymeric scaffolds,” European Journal of Histochemistry, vol. 51, no. 1, pp. 1–8, 2007. View at Scopus
  5. D. Foudah, S. Redaelli, E. Donzelli et al., “Monitoring the genomic stability of in vitro cultured rat bone-marrow-derived mesenchymal stem cells,” Chromosome Research, vol. 17, no. 8, pp. 1025–1039, 2009. View at Publisher · View at Google Scholar · View at Scopus
  6. E. Karaoz, A. Aksoy, S. Ayhan, A. E. SarIboyacI, F. Kaymaz, and M. Kasap, “Characterization of mesenchymal stem cells from rat bone marrow: ultrastructural properties, differentiation potential and immunophenotypic markers,” Histochemistry and Cell Biology, vol. 132, no. 5, pp. 533–546, 2009. View at Publisher · View at Google Scholar · View at Scopus
  7. B. Neuhuber, G. Gallo, L. Howard, L. Kostura, A. Mackay, and I. Fischer, “Reevaluation of in vitro differentiation protocols for bone marrow stromal cells: disruption of actin cytoskeleton induces rapid morphological changes and mimics neuronal phenotype,” Journal of Neuroscience Research, vol. 77, no. 2, pp. 192–204, 2004. View at Publisher · View at Google Scholar · View at Scopus
  8. Z. Lei, L. Yongda, M. Jun et al., “Culture and neural differentiation of rat bone marrow mesenchymal stem cells in vitro,” Cell Biology International, vol. 31, no. 9, pp. 916–923, 2007. View at Publisher · View at Google Scholar · View at Scopus
  9. G. F. Barnabé, T. T. Schwindt, M. E. Calcagnotto et al., “Chemically-induced RAT mesenchymal stem cells adopt molecular properties of neuronal-like cells but do not have basic neuronal functional properties,” PLoS ONE, vol. 4, no. 4, Article ID e5222, 2009. View at Publisher · View at Google Scholar · View at Scopus
  10. M. G. Thomas, L. Stone, L. Evill, S. Ong, M. Ziman, and L. Hool, “Bone marrow stromal cells as replacement cells for Parkinson's disease: generation of an anatomical but not functional neuronal phenotype,” Translational Research, vol. 157, no. 2, pp. 56–63, 2011. View at Publisher · View at Google Scholar · View at Scopus
  11. S. Wislet-Gendebien, P. Leprince, G. Moonen, and B. Rogister, “Regulation of neural markers nestin and GFAP expression by cultivated bone marrow stromal cells,” Journal of Cell Science, vol. 116, no. 16, pp. 3295–3302, 2003. View at Publisher · View at Google Scholar · View at Scopus
  12. P. Lu, A. Blesch, and M. H. Tuszynski, “Induction of bone marrow stromal cells to neurons: differentiation, transdifferentiation, or artifact?” Journal of Neuroscience Research, vol. 77, no. 2, pp. 174–191, 2004. View at Publisher · View at Google Scholar · View at Scopus
  13. A. P. Croft and S. A. Przyborski, “Formation of neurons by non-neural adult stem cells: potential mechanism implicates an artifact of growth in culture,” Stem Cells, vol. 24, no. 8, pp. 1841–1851, 2006. View at Publisher · View at Google Scholar · View at Scopus
  14. G. E. Rooney, L. Howard, T. O'Brien, A. J. Windebank, and F. P. Barry, “Elevation of cAMP in mesenchymal stem cells transiently upregulates neural markers rather than inducing neural differentiation,” Stem Cells and Development, vol. 18, no. 3, pp. 387–398, 2009. View at Publisher · View at Google Scholar · View at Scopus
  15. G. Nicolini, R. Rigolio, A. Scuteri et al., “Effect of trans-resveratrol on signal transduction pathways involved in paclitaxel-induced apoptosis in human neuroblastoma SH-SY5Y cells,” Neurochemistry International, vol. 42, no. 5, pp. 419–429, 2003. View at Publisher · View at Google Scholar · View at Scopus
  16. F. Ronca, S. L. Chan, and V. C. Yu, “1-(5-Isoquinolinesulfonyl)-2-methylpiperazine induces apoptosis in human neuroblastoma cells, SH-SY5Y, through a p53-dependent pathway,” Journal of Biological Chemistry, vol. 272, no. 7, pp. 4252–4260, 1997. View at Publisher · View at Google Scholar · View at Scopus
  17. M. Dominici, K. Le Blanc, I. Mueller et al., “Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement,” Cytotherapy, vol. 8, no. 4, pp. 315–317, 2006. View at Publisher · View at Google Scholar · View at Scopus
  18. C. D. Katsetos, A. Legido, E. Perentes, and S. J. Mörk, “Class III β-tubulin isotype: a key cytoskeletal protein at the crossroads of developmental neurobiology and tumor neuropathology,” Journal of Child Neurology, vol. 18, no. 12, pp. 851–866, 2003. View at Scopus
  19. R. J. Mullen, C. R. Buck, and A. M. Smith, “NeuN, a neuronal specific nuclear protein in vertebrates,” Development, vol. 116, no. 1, pp. 201–211, 1992. View at Scopus
  20. M. K. Lee and D. W. Cleveland, “Neuronal intermediate filaments,” Annual Review of Neuroscience, vol. 19, pp. 187–217, 1996. View at Scopus
  21. L. F. Eng, R. S. Ghirnikar, and Y. L. Lee, “Glial fibrillary acidic protein: GFAP-thirty-one years (1969–2000),” Neurochemical Research, vol. 25, no. 9-10, pp. 1439–1451, 2000. View at Scopus
  22. R. Donato, “S100: a multigenic family of calcium-modulated proteins of the EF-hand type with intracellular and extracellular functional roles,” International Journal of Biochemistry and Cell Biology, vol. 33, no. 7, pp. 637–668, 2001. View at Publisher · View at Google Scholar · View at Scopus
  23. J. Sodek, B. Ganss, and M. D. McKee, “Osteopontin,” Critical Reviews in Oral Biology and Medicine, vol. 11, no. 3, pp. 279–303, 2000. View at Scopus
  24. P. V. Hauschka, “Osteocalcin: the vitamin K-dependent Ca2+-binding protein of bone matrix,” Haemostasis, vol. 16, no. 3-4, pp. 258–272, 1986. View at Scopus
  25. E. D. Rosen and B. M. Spiegelman, “PPARγ: a nuclear regulator of metabolism, differentiation, and cell growth,” Journal of Biological Chemistry, vol. 276, no. 41, pp. 37731–37734, 2001. View at Scopus
  26. C. Wiese, A. Rolletschek, G. Kania et al., “Nestin expression—a property of multi-lineage progenitor cells?” Cellular and Molecular Life Sciences, vol. 61, no. 19-20, pp. 2510–2522, 2004. View at Publisher · View at Google Scholar · View at Scopus
  27. B. K. Dredge and K. B. Jensen, “NeuN/Rbfox3 Nuclear and Cytoplasmic isoforms differentially regulate alternative splicing and nonsense-mediated decay of Rbfox2,” PLoS ONE, vol. 6, no. 6, Article ID e21585, 2011. View at Publisher · View at Google Scholar · View at Scopus
  28. N. R. Blondheim, Y. S. Levy, T. Ben-Zur et al., “Human mesenchymal stem cells express neural genes, suggesting a neural predisposition,” Stem Cells and Development, vol. 15, no. 2, pp. 141–164, 2006. View at Publisher · View at Google Scholar · View at Scopus
  29. T. Tondreau, L. Lagneaux, M. Dejenefle et al., “Bone marrow-derived mesenchymal stem cells already express specific neural proteins before any differentiation,” Differentiation, vol. 72, no. 7, pp. 319–326, 2004. View at Publisher · View at Google Scholar · View at Scopus
  30. Y. Takashima, T. Era, K. Nakao et al., “Neuroepithelial cells supply an initial transient wave of MSC differentiation,” Cell, vol. 129, no. 7, pp. 1377–1388, 2007. View at Publisher · View at Google Scholar · View at Scopus
  31. S. Morikawa, Y. Mabuchi, K. Niibe et al., “Development of mesenchymal stem cells partially originate from the neural crest,” Biochemical and Biophysical Research Communications, vol. 379, no. 4, pp. 1114–1119, 2009. View at Publisher · View at Google Scholar · View at Scopus
  32. C. J. Schoenherr and D. J. Anderson, “The neuron-restrictive silencer factor (NRSF): a coordinate repressor of multiple neuron-specific genes,” Science, vol. 267, no. 5202, pp. 1360–1363, 1995. View at Scopus
  33. F. M. J. Lamoury, J. Croitoru-Lamoury, and B. J. Brew, “Undifferentiated mouse mesenchymal stem cells spontaneously express neural and stem cell markers Oct-4 and Rex-1,” Cytotherapy, vol. 8, no. 3, pp. 228–242, 2006. View at Publisher · View at Google Scholar · View at Scopus
  34. L. Fu, L. Zhu, Y. Huang, T. D. Lee, S. J. Forman, and C. C. Shih, “Derivation of neural stem cells from mesenchymal stem cells: evidence for a bipotential stem cell population,” Stem Cells and Development, vol. 17, no. 6, pp. 1109–1121, 2008. View at Publisher · View at Google Scholar · View at Scopus