Table 1: Undifferentiated rMSC expression of differentiation markers at several passages after 14 days of culture. The number of positive cells for each marker is expressed as % ± SD (—: no positive cells). P80 is not reported in the table because rMSCs are always negative for all markers.

MarkerPassage Significant differences (ANOVA)
P0P1P2P4P8P16 P24 P40

Nestin55 ± 21,252,5 ± 24,826,25 ± 1,856 ± 5,561,3 ± 11,168,3 ± 10,428,8 ± 1,83,5 ± 0,7 value < 0.001 P16 versus P40.
value < 0.01 P0 versus P40;
P1 versus P40;
P4 versus P40;
P8 versus P40.
value < 0.05 P2 versus P16;
P16 versus P24.

III tubulin36,5 ± 2,172,5 ± 3,564,2 ± 3,8 56,7 ± 7,630 ± 8,211,7 ± 3,812,5 ± 3,50,9 ± 0,14 value < 0.001 P0 versus P1, P2, P16, P24, P40;
P1 versus P8, P16, P24, P40;
P2 versus P8, P16, P40;
P4 versus P8, P16, P40;
P8 versus P40.
value < 0.01 P0 versus P4;
P8 versus P16, P24.
value < 0.05 P1 versus P4.

NeuN36 ± 1,418,8 ± 1,847,5 ± 17,750 ± 8,720 ± 77 ± 4,2 0,9 ± 0,14 value < 0.001 P0 versus P24, P40;
P2 versus P16, P24, P40;
P4 versus P16, P24, P40.
value < 0.01 P0 versus P16;
P1 versus P2, P4;
P2 versus P8;
P4 versus P8.

NF0,9 ± 0,11 0,8 ± 0,140,85 ± 0,100,88 ± 0,160,85 ± 0,21 value < 0.001 P0 versus all except P1 and P40;
P1 versus all except P0 and P40;
P2 versus P40;
P4 versus P40;
  P8 versus P40;
P16 versus P40;
P24 versus P40.

GFAP55 ± 7,119 ± 7,111 ± 2,87,2 ± 311 ± 4,25,8 ± 2,53 ± 1,4 value < 0.001 P0 versus all;
P1 versus P40.
value < 0.01 P1 versus P24.
value < 0.05 P1 versus P16.

S10010,3 ± 0,43,8 ± 1,80,95 ± 0,070,93 ± 0,10,98 ± 0,040,9 ± 0,040,93 ± 0,04 value < 0.001 P0 versus all;
P1 versus P40.
value < 0.01 P1 versus P2, P4, P8, P16, P24.

PPAR 23,9 ± 0,144,25 ± 0,352,45 ± 0,070,78 ± 0,040,68 ± 0,110,8 ± 0,140,85 ± 0,210,83 ± 0,11 value < 0.001 P0 versus all except P1;
P1 versus all except P0;
P2 versus all.

OPN12,2 ± 0,411 ± 5,725 ± 14,110 ± 2,89,75 ± 3,95,25 ± 3,20,9 ± 0,10,83 ± 0,2 value < 0.01 P2 versus P24, P40.
value < 0.05 P2 versus P16.