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Journal of Biomedicine and Biotechnology
Volume 2012 (2012), Article ID 823949, 6 pages
Research Article

Detection of Herplex Simplex Virus-1 and -2 in Cardiac Myxomas

1Molecular Carcinogenesis Group, Department of Histology and Embryology, School of Medicine, National and Kapodistrian University of Athens, 11527, Greece
2First Department of Pathology, “Laiko” University Hospital, School of Medicine, National and Kapodistrian University of Athens, 11527 Athens, Greece
3Department of Cytopathology, “Attikon” University Hospital, 12464 Athens, Greece
4Experimental-Research Center, ELPEN Pharma, 19009 Pikermi, Greece

Received 13 November 2011; Accepted 26 November 2011

Academic Editor: Aristides Eliopoulos

Copyright © 2012 Ioannis S. Pateras et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The etiology of sporadic cardiac myxomas remains elusive. The tendency for these lesions to recur following resection, their immunopathological characteristics, along with their histological and molecular profile, may implicate the presence of an infective agent in this type of tumor. In this study, we investigated the presence of herpes simplex virus (HSV) DNA in a cohort of cardiac myxomas in a tertiary referral centre. Twenty-nine formalin-fixed paraffin-embedded (FFPE) sporadic cardiac myxomas were obtained, 17 of which were shown to be informative. These were compared to 19 macroscopically and microscopically normal heart tissue specimens. The detection of HSV-1 and -2 genomic sequences was achieved with the use of a combined nested PCR-Restriction Fragment Length Polymorphism methodology. The presence of HSV-1 and/or -2 DNA was demonstrated in 6 of 17 (35%) informative sporadic cardiac myxomas, whereas no HSV DNA was detected in normal heart tissues ( 𝑃 < 0 . 0 1 ). The existence of HSV-1/2 DNA in sporadic cardiac myxomas, along with its absence from normal heart tissues, reinforces the possibility that HSV infection might be involved in the development of these lesions. Our findings raise the point of anti-HSV medication postsurgically with a potential benefit in reducing the rate of recurrences.