Prevention of Chronic Experimental Colitis Induced by Dextran Sulphate Sodium (DSS) in Mice Treated with FR91

Figure 3

Immunohistochemistry of colorectal pathological markers in different transverse section of colon in mice. (a–e) Details of transverse sections at middle and distal colorectal levels of mice group 1, showing scarce immunoreactivity to Catenin-β, p53 or absence of BCL-2, MLH-1, and APC. Note that these sections also show a normal epithelial organization with well-differentiated cryptal cells. (f–j) Details of transverse colon sections of mice group 2, showing intense immunoreactivity to the cell markers studied. Note the numerous immunoreactive cells (arrows in (f–i)) and their massive location at the different pathological lesions, such as severe-grade dysplasia (arrowheads in (f)), polyps (arrowhead in (j)), and adenomas. (k–o) Details of transverse colon sections of mice group 3, showing moderate-to-scarce immunoreactivity to the cell markers in the colorectal segments. Note some immunoreactive cells to Catenin-β (arrowheads in (k)), MLH-1 (arrow in (m)), APC (arrows in (n)), and p53 (arrows in (o)). (p–t) and (u–y). Details of transverse colon sections of mice group 4 (p–t) and goup 5 (u–y), showing absence or basal (arrows in (s) and (x)) immunoreactivity to the cell markers in the colorectal segments studied. Scale bar: 100 μm.

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