Comparison of Two Suspension Arrays for Simultaneous Detection of Five Biothreat Bacterial in Powder Samples

Table 1

The primers and probes for detection of Bacillus anthracis, Francisella tularensis, Yersinia pestis, Brucella spp. and Burkholderia pseudomallei by multiplex PCR suspension arrays.

Target organism	Name	Sequence (5′-3′)	Gene location	Product size
Bacillus anthracis	BA-1-F	TGGACGCATACGAGACATAAT	capB	430 bp
	BA-1-R	TGCTTTAGCGGTAGCAGAGG
	BA-1-P	GAAGAACGCAGGCTTAGATTGGT
	BA-2-F	TTTCATAATCATGGATTTCCCG	chromosome	212 bp
	BA-2-R	TTACCCAACATCATCTTCGCA
	BA-2-P	CTCGCTTTCATCGCATTTCTCCC
Brucella spp.	Bru-F	TGGCTCGGTTGCCAATATCAA	BCSP31	223 bp
	Bru-R	GCGCTTGCCTTTCAGGTCTG
	Bru-P	TTACGCAGTCAGACGTTGCCTAT
Francisella tularensis	FT-F	GGGCAAATCTAGCAGGTCAAG	fopA	250 bp
	FT-R	GCTGTAGTCGCACCATTATCCT
	Ft-P	TGCTGGTTTAACATGGTTCTTTGG
Yersinia pestis	YP-F	ACTCAATGTTGTGACGAGGATG	chromosome	220 bp
	YP-R	TTACTTCTAATGCCATCAGGTAGC
	Yp-P	AACAGTAAGCATCCAGTCGTTCATA
Burkholderia pseudomallei	BP-F	CGATCTCGTCAAGGTGTCGG	chromosome	150 bp
	BP-R	CCCCAGTTCATCTGATACTTGC
	Bp-P	AGGTCAATTTCCCGAACAAGACT