Table 1: The primers and probes for detection of Bacillus anthracis, Francisella tularensis, Yersinia pestis, Brucella spp. and Burkholderia pseudomallei by multiplex PCR suspension arrays.

Target organism Name Sequence (5′-3′) Gene location Product size Bacillus anthracis BA-1-F TGGACGCATACGAGACATAAT capB 430 bp BA-1-R TGCTTTAGCGGTAGCAGAGG BA-1-P GAAGAACGCAGGCTTAGATTGGT BA-2-F TTTCATAATCATGGATTTCCCG chromosome 212 bp BA-2-R TTACCCAACATCATCTTCGCA BA-2-P CTCGCTTTCATCGCATTTCTCCC Brucella spp. Bru-F TGGCTCGGTTGCCAATATCAA BCSP31 223 bp Bru-R GCGCTTGCCTTTCAGGTCTG Bru-P TTACGCAGTCAGACGTTGCCTAT Francisella tularensis FT-F GGGCAAATCTAGCAGGTCAAG fopA 250 bp FT-R GCTGTAGTCGCACCATTATCCT Ft-P TGCTGGTTTAACATGGTTCTTTGG Yersinia pestis YP-F ACTCAATGTTGTGACGAGGATG chromosome 220 bp YP-R TTACTTCTAATGCCATCAGGTAGC Yp-P AACAGTAAGCATCCAGTCGTTCATA Burkholderia pseudomallei BP-F CGATCTCGTCAAGGTGTCGG chromosome 150 bp BP-R CCCCAGTTCATCTGATACTTGC Bp-P AGGTCAATTTCCCGAACAAGACT