Research Article

Comparison of Two Suspension Arrays for Simultaneous Detection of Five Biothreat Bacterial in Powder Samples

Table 2

The Universal primers and probes for detection of Bacillus anthracis, Francisella tularensis, Yersinia pestis, Brucella spp. and Burkholderia pseudomallei by suspension arrays.

Target organismNameSequence (5′-3′)

16S rRNA universal primers341a CCTACGGGAGGCAGCAGT
519b ATTACCGCGGC (T/G) GCTG
Bacillus anthracisB.a AAGTGCTAGTTGAATAAGCTGGCAC
Brucella spp.Bru GGAGAAGATAATGACGGTAACCCGA
Francisella tularensisF.t GCCTCAAGGTTAATAGCCTTGGGGA
Yersinia pestisY.p AAGGGGTTGAGTTTAATACGCTCAA
Burkholderia pseudomalleiB.p AATCATTCTGGCTAATACCCGGAGT