Identification of HLA-A24-Restricted Novel T Cell Epitope Peptides Derived from P-Cadherin and Kinesin Family Member 20A
Figure 1
IFN-γ production from CTLs responding to CDH3- or KIF20A-derived peptides. IFN-γ production by CTLs induced with CDH3-derived peptides (a) or KIF20A-derived peptides (b) responding to respective peptide-pulsed HLA-A*2402 positive TISI cells. CTLs were expanded and harvested following “in vitro induction of peptide-specific CTL,” and IFN-γ production was examined by IFN-γ ELISA. “Closed bar” indicates the mean IFN-γ production responding to TISI cells pulsed with indicated peptide, and “open bar” indicates the mean IFN-γ production responding to TISI cells pulsed with HIV-A24 peptide (negative control). All experiments were performed triplicate. Similar results were obtained in three to five independent experiments. *, **.
