Identification of HLA-A24-Restricted Novel T Cell Epitope Peptides Derived from P-Cadherin and Kinesin Family Member 20A
Figure 4
Peptide-specific TCR expression on CTL clones. (a) CDH3-10-807/HLA-A*2402-specific TCR expressing cells in expanded CTLs following “in vitro induction of peptide-specific CTL.” (b) KIF20A-10-66/HLA-A*2402-specific TCR expressing cells in expanded CTLs following “in vitro induction of peptide-specific CTL.” Results staining with anti-human CD8 mAb and CDH3-10-807/MHC-dextramer-PE or KIF20A-10-66/MHC-dextramer-PE are presented following gating on CD3-positive cells (left panels). Results staining with anti-human CD8 mAb and HIV-A24/MHC-dextramer-PE are presented as negative control following gating on CD3 positive cells (right panels). (c) CDH3-10-807/HLA-A*2402-specific TCR expression on CDH3-10-807-specific CTL clone. (d) KIF20A-10-66/HLA-A*2402-specific TCR expression on KIF20A-10-66-specific CTL clone. Staining with HIV-A24/MHC-dextramer-PE was used as negative control. CTL clones were CD3+ and CD8+ as expected (data not shown). Similar results were obtained in independent all experiments to examine CTL induction.
