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Journal of Biomedicine and Biotechnology
Volume 2012 (2012), Article ID 856324, 5 pages
http://dx.doi.org/10.1155/2012/856324
Research Article

The Effect of Glycyrrhetinic Acid on Pharmacokinetics of Cortisone and Its Metabolite Cortisol in Rats

1Analytical and Testing Center, Wenzhou Medical College, Wenzhou 325035, China
2The 2nd Affiliated Hospital Wenzhou Medical College, Wenzhou 325027, China
3School of Pharmacy, Wenzhou Medical College, Wenzhou 325035, China
4Teaching Center of Medical Functional Experiment, Wenzhou Medical College, Wenzhou 325035, China

Received 2 July 2012; Revised 1 October 2012; Accepted 2 October 2012

Academic Editor: James D. Murray

Copyright © 2012 Dan Lin et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The purpose of this paper is to study pharmacokinetics of cortisone (E) and its metabolite cortisol (F) in rats after administration of glycyrrhetinic acid (GA) and cortisone. Healthy male SD rats were randomized to be given 20 mg/kg E or E combined with 10 mg/kg GA. Blood samples were collected at 5, 10, 20, 40, 60, 90, 120, 150, 180, and 240 min after administration. The serum concentrations of E and F were determined by HLPC and pharmacokinetic parameters were calculated using DASver2.0 software. The parameters of , , and for E in the group of E + GA were significantly higher than those in the group of E ( ); the half-time ( ) was extended compared to E ( ) and CL/F was dropped obviously ( ). The rise in , , and for cortisol in the group of E + GA was significantly compared to the group of E ( ). CL/F was lower than E ( ) and the half-time ( ) was slightly extended. In this study, we find that GA restrains the metabolism of E and F and thus increases AUC, , and of E and F, which may be related to its inhibition effect on 11β-hydroxysteroid dehydrogenase (11β-HSD).