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867023.fig.001b
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Figure 1: Cell numbers and morphology changes of F4/ 8 0 + RPMs in 5-month db/db mice. Purity of F4/ 8 0 + RPMs was determined by FCM (a). The number of F4/ 8 0 + RPMs was calculated by multiplying the total number of trypan blue negative cells by the ratio of F4/80 positive cells. The average number of RPMs of six db/db or control mice was caculated (b), and the mean value of standardized RPM numbers with body weight from six db/db or control mice was taken (c). Morphology of RPMs was assayed by Wright-Giemsa staining or double-immunofluorescence staining and laser confocal scanning microscopy. RPMs from control mice (d) and diabetic mice (e) were stained by Wright-Giemsa; (f) and (g) were F4/ 8 0 + RPMs from control or diabetic mice, respectively, assayed by double-immunofluorescence staining and laser confocal scanning microscopy. Scale bars represent 50 μm. **P < 0.01 between the indicated groups.