Generation of a Chinese Hamster Ovary Cell Line Producing Recombinant Human Glucocerebrosidase

Figure 5

Enzymatic activity of recombinant GCR secreted from CHO-DXB11 cell subclones (SC). The reactions were carried out with the synthetic fluorogenic substrate 4-MUG. The product of enzymatic reactions 4-MU was measured at wavelength of 445 nm after excitation at 365 nm. A standard curve (units per fluorescence) was constructed using specific activity data of Cerezyme. Results are specific activities obtained for subclones relative to Cerezyme, presented as means ± SEM, measured in triplicate. The volume of conditioned medium used in this assay corresponded to the supernatant of 70,000 cells.