Table 1: Particular form of proteins with a different abundance from T. cruzi epimastigotes subjected to heat shock identified by MALDI-TOF.

Particular form of a protein identitySpotAccession numberTheoretical pl/MW (kDa)Experimental pl/MW (kDa)Sequence coverage (%)MOWSE scoreAbundance (folds relative to control)

Metabolism
Transitional endoplasmic reticulum ATPase, putative3Q4DWB55.4/86.15.4/7750.52.057
ATP synthase, putative7Q4E1T66.0/53.75.9/57.622.81011.876
Glutamate dehydrogenase, putative*13Q4CR186.0/43.26.1/43.424.516.81.317
Calcium translocating P-type ATPase*16Q86QH68.3/37.38.2/37.928.262.11.197
NAD-/FAD-dependent dehydrogenase, putative11Q4CVH05.3/43.05.3/43.421.472.61.072
Aromatic L-α-hydroxy acid dehydrogenase17Q9NJT26.8/33.76.9/34.125.3204−1.228

Cell defense
Heat shock protein 70 (HSP70), putative6Q4DTM95.8/70.95.8/63.429.756111.776
Thiol transferase Tc5210Q7Z0C85.4/48.95.5/48.550.54736011.544
Tryparedoxin peroxidase, putative22Q4CX877.6/25.57.8/25.635.01241.125
Chaperone DNAJ protein, putative19Q4E2447.6/35.37.6/34.941.723491.065

Interaction with the cellular environment
Surface glycoprotein Tc-85/16*8Q2VYD05.5/66.85.6/61.527.61.811.821
Mucin-associated surface protein (MASP), putative9Q4CYQ54.4/50.04.2/5329.36921.403
Surface protease gp63, putative5Q4DTZ35.7/78.65.6/6646.11.218
Mucin-associated surface protein (MASP), putative*23Q4DQ684.8/26.54.8/26.645.329.4−1.514

Hypothetical protein
Hypothetical protein1Q4D9E45.6/88.35.7/82.318.911601.577
Hypothetical protein15Q4CYM68.4/39.28.5/39.441.211971.569
Hypothetical protein14Q4CTH88.2/39.58.2/39.424.357131.537
Hypothetical protein20Q4E5C78.7/34.88.7/34.644.33723−1.114

Protein fate
Ubiquitin hydrolase, putative4Q4E6805.6/81.15.5/71.438.61.390
Ubiquitin-conjugating enzyme E2, putative24Q4DVH24.5/25.74.5/2634.896.6−1.293
Proteasome regulatory non-ATPase subunit, putative*18Q4CPL06.0/25.26.1/25.741.77.13−1.226

Protein synthesis
Cysteinyl-tRNA synthetase, putative*2Q4CQ346.1/89.26.2/85.11328.91.396

Cellular transport
Rab6 GTPase-activating protein, putative12Q4DHN95.8/44.25.8/44.325.747391.342

Cell cycle
Retrotransposon hot-spot (RHS) protein, putative21Q4D6R88.6/29.08.7/28.836.114561.206

*These proteins were not discussed because their MOWSE score is lower than 67.