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Journal of Biomedicine and Biotechnology
Volume 2012 (2012), Article ID 928159, 7 pages
http://dx.doi.org/10.1155/2012/928159
Research Article

The Inhibitory Effect of Prunella vulgaris L. on Aldose Reductase and Protein Glycation

1Institute of Natural Medicine, Hallym University, Chuncheon 200-702, Republic of Korea
2Research Institute, S&D Co., Ltd., Chuncheon 200-161, Republic of Korea
3Agricultural College, Yanbian University, Yanji 133-002, China
4Department of Food Science and Nutrition, Hallym University, Chuncheon 200-702, Republic of Korea

Received 8 May 2012; Accepted 25 July 2012

Academic Editor: George Perry

Copyright © 2012 Hong Mei Li et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

To evaluate the aldose reductase (AR) enzyme inhibitory ability of Prunella vulgaris L. extract, six compounds were isolated and tested for their effects. The components were subjected to in vitro bioassays to investigate their inhibitory assays using rat lens aldose reductase (rAR) and human recombinant AR (rhAR). Among them, caffeic acid ethylene ester showed the potent inhibition, with the IC50 values of rAR and rhAR at 3 . 2 ± 0 . 5 5 μM and 1 2 . 5 8 ± 0 . 3 2 μM, respectively. In the kinetic analyses using Lineweaver-Burk plots of 1/velocity and 1/concentration of substrate, this compound showed noncompetitive inhibition against rhAR. Furthermore, it inhibited galactitol formation in a rat lens incubated with a high concentration of galactose. Also it has antioxidative as well as advanced glycation end products (AGEs) inhibitory effects. As a result, this compound could be offered as a leading compound for further study as a new natural products drug for diabetic complications.