Sunflower Oil Supplementation Has Proinflammatory Effects and Does Not Reverse Insulin Resistance in Obesity Induced by High-Fat Diet in C57BL/6 Mice

Table 3

Adipokine production in adipose tissue from mice fed with control diet (CD) or high-fat diet (HFD), supplemented, or not with sunflower oil (rich in n-6 fatty acids).

	CD	HFD	CD + n6	HFD + n6
Epididymal adipose tissue^a
Leptin	42.51 ± 8.75	81.38±9.90^∗	62.42 ± 8.80	88.03 ± 10.96
Adiponectin	659.9 ± 39.94	697.6 ± 43.85	730.3 ± 76.68	649.8 ± 45.38
Resistin	625.1 ± 100.8	528.6 ± 71.65	913.0 ± 181.0	327.0 ± 42.15^#

Oral supplementation with n-6 (2 g/Kg body weight, three times a week, gavage) or water started four weeks before HFD and continued until the end of experiment. Animals were feed with HFD or CD for eight weeks. Approximately 150 mg of tissue were incubated in DMEM medium for 24 h and the supernatant were used for measures. Mean ± SEM (𝑛 = 7–11). ANOVA two-way with Bonferroni post tests. ^∗𝑃<0.05 when compared with CD; ^#𝑃<0.05 when compared with CD + n6. ^aValues are expressed as pg/mL/mg tissue.