Effects of ginsenoside Rb1 on Bax, Bak, and Bcl-2 expression levels in UVB-irradiated human primary keratinocytes [31]. Human keratinocytes (3 × 105 cells) were seeded in a 100-mm culture dish and cultured in KG-2 medium for 48 h. The cells were irradiated with UVB (20 mJ/cm2) and treated with the indicated amounts of ginsenoside Rb1 for 24 h in KB-2 medium. After being washed with phosphate-buffered saline (PBS, pH 7.0), the cells were treated with lysed buffer. The supernatant obtained by centrifugation was subjected to a western blot analysis with anti-Bcl-2, anti-Bax, anti-Bak, and anti-β-actin antibodies.
