Effects of ginsenoside Rb1 and bFGF on IL-1Ξ² production in the exudates of burns in male Balb/c mice [26]. The burn wounds were created on the backs of male Balb/c mice (6 weeks old) under anesthesia with pentobarbital. A polyethylene filter pellet (about 8 in mm diameter, 3βmm thick) containing the indicated amount of basic fibroblast growth factor (bFGF) or ginsenoside Rb1 was applied to the burn wounds surface. On days 1, 3, 5, 7, and 9, the filter pellets were removed and replaced with fresh filter pellets. For control mice, filter pellets containing saline alone were applied according to the same schedule. Immediately after removal, phosphate-buffered saline (PBS, pH 7.0) (200βΞΌl) was added to each filter pellet and mixed for 10βmin. The IL-1Ξ² levels in the filter pellets were measured using a mouse IL-1Ξ² ELISA kit. Values are the mean Β± SE for 6 mice. *Significantly different from control, .
