Effects of Ginsenoside 𝐑𝐛_𝟏 on Skin Changes

Table 4

Effects of ginsenoside Rb₁ on the thickness of the epidermis and extracellular matrix (ECM) of the dermis at week 12 in UVB-irradiated hairless mice [31].

	Epidermis (μm)	ECM (μm) in dermis
Normal mice	14.74±1.11 *	332.51±23.18 *
Vehicle-treated UVB-irradiated mice (control)	142.59±25.37	632.32±31.96
+Ginsenoside Rb1
(100 fg/mouse)	46.00±6.26 *	561.86±45.22
(10 pg/mouse)	49.24±4.73 *	560.67±44.81
(1 ng/mouse)	39.84±6.26 *	585.63±31.35

The initial dose of UVB was set at 36 mJ/cm², which was subsequently increased to 54 mJ/cm² at weeks 1–4, 72 mJ/cm² at weeks 4–7, 108 mJ/cm² at weeks 7–10, and finally to 122 mJ/cm² at weeks 10–12 in male albino hairless HOS: HR-1 mice. The frequency of UVB irradiation was set at three times per week. Ginsenoside Rb₁ (100 fg, 10 pg, and 1 ng/mouse) was applied topically to the dorsal region of each mouse every day for 12 weeks. The dorsal skin samples (about 3 cm²) removed at week 12 were fixed in 10% buffered formalin, embedded in paraffin, sectioned at 5 μm thickness, deparaffinized, and stained with hematoxylin-eosin (HE) and Azan. Four different microscopic fields (×200 magnification) per plate were photographed. The thickness of the epidermis and dermis thickness were measured from the samples stained by HE and Azan, using a Digimatic Caliper.
Values are the mean ± SE for 6 mice. *Significantly different from UVB-irradiated hairless mice (control), 𝑃<0.05.