Research Article
Characterization, Tissue Expression, and Imprinting Analysis of the Porcine CDKN1C and NAP1L4 Genes
Table 1
Primer sequences and their information for porcine CDKN1C and NAP1L4 gene.
| Genes | Primer application | Primer name | Primer sequence (5′ → 3′) | Tm (°C) | Product size (bp) |
| CDKN1C | Gene cloning | CDF1 | TTCTACCGCGAGACGGTG | 56 | 776, cDNA | CDR1 | AGCTTTACACCTTGGGACC | Intron amplification | INF1 | CAAGGCGTCGAACGAGGTTC | 61.4 | 331, DNA | INR1 | ACAAAACCGAACGCTGCTCC | IMpRH | MF1 | TGGCTCACCGCACCTCTCTCCC | 60 | 222, DNA | MR1 | CTCCTCGTGCAGAATACATTTAG | Imprinting assay | IJF1 | CTCCGTGCCCGCCTTCTACCG | 66.4 | 1459, DNA and 694, cDNA | IJR1 | AAAACCGAACGCTGCTCCGTGG | 5′RACE | 5′CDGSP1 | GAAATCCCTGAGTGCGGCGGCTC | 60 | 690, cDNA | 5′CDGSP2 | CCACCTGCACCGTCTCGCGGTAG | 60 | 443, cDNA | Real-time PCR | QF1 | ACGAGGAGCTGAGTCGTGAG | 60 | 136, cDNA | QR1 | CTGTCCACCTCGGTCCACT |
| NAP1L4 | Gene cloning | NAF1 | GCAGGAGGCGTGAGGAGAT | 55.2 | 917, cDNA | NAR1 | CGGGCTGAAGAAGTTGAAGA | Intron amplification | INF2 | GCAGCGAAGCGAAGAATCAAC | 56.6 | 368, cDNA | INR2 | CTCAACGTCACCCGTGATGAAC | IMpRH | MF2 | GCACGTCTCTGCTCCTGCAG | 60 | 196, cDNA | MR2 | CACCCGTGATGAACTCCCTTC | Imprinting assay | IJF2 | GGAGTGCGAGCAGCAGTGAGA | 60.7 | 963, cDNA | IJR2 | TCAGTTACAAACGCATCGTGAGG | 3′RACE | 3′NAGSP1 | GGTGCACCATTGACTGGAAG | 55 | 1440, cDNA | 3′NAGSP2 | CGTGACGGTGAAAACCATC | 55 | 1409, cDNA | 5′RACE | 5′NAGSP1 | CCTGCAAAGCCGCCAGAACTTGAG | 60 | 591, cDNA | 5′NAGSP2 | GACTCTGAAGGAACCCCATCTG | 60 | 511, cDNA | Real-time PCR | QF2 | GACTGGAAGAAAGGCAAGAACG | 60 | 128, cDNA | QR2 | GGGCTGAAGAAGTTGAAGAAGG |
| RPL32 | Real-time PCR | QF3 | AGCCCAAGATCGTCAAAAAG | 60 | 165, cDNA | QR3 | TGTTGCTCCCATAACCAATG |
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