Characterization, Tissue Expression, and Imprinting Analysis of the Porcine CDKN1C and NAP1L4 Genes

Table 1

Primer sequences and their information for porcine CDKN1C and NAP1L4 gene.

Genes	Primer application	Primer name	Primer sequence (5′ → 3′)	Tm (°C)	Product size (bp)
CDKN1C	Gene cloning	CDF1	TTCTACCGCGAGACGGTG	56	776, cDNA
		CDR1	AGCTTTACACCTTGGGACC
	Intron amplification	INF1	CAAGGCGTCGAACGAGGTTC	61.4	331, DNA
		INR1	ACAAAACCGAACGCTGCTCC
	IMpRH	MF1	TGGCTCACCGCACCTCTCTCCC	60	222, DNA
		MR1	CTCCTCGTGCAGAATACATTTAG
	Imprinting assay	IJF1	CTCCGTGCCCGCCTTCTACCG	66.4	1459, DNA and 694, cDNA
		IJR1	AAAACCGAACGCTGCTCCGTGG
	5′RACE	5′CDGSP1	GAAATCCCTGAGTGCGGCGGCTC	60	690, cDNA
		5′CDGSP2	CCACCTGCACCGTCTCGCGGTAG	60	443, cDNA
	Real-time PCR	QF1	ACGAGGAGCTGAGTCGTGAG	60	136, cDNA
		QR1	CTGTCCACCTCGGTCCACT
NAP1L4	Gene cloning	NAF1	GCAGGAGGCGTGAGGAGAT	55.2	917, cDNA
		NAR1	CGGGCTGAAGAAGTTGAAGA
	Intron amplification	INF2	GCAGCGAAGCGAAGAATCAAC	56.6	368, cDNA
		INR2	CTCAACGTCACCCGTGATGAAC
	IMpRH	MF2	GCACGTCTCTGCTCCTGCAG	60	196, cDNA
		MR2	CACCCGTGATGAACTCCCTTC
	Imprinting assay	IJF2	GGAGTGCGAGCAGCAGTGAGA	60.7	963, cDNA
		IJR2	TCAGTTACAAACGCATCGTGAGG
	3′RACE	3′NAGSP1	GGTGCACCATTGACTGGAAG	55	1440, cDNA
		3′NAGSP2	CGTGACGGTGAAAACCATC	55	1409, cDNA
	5′RACE	5′NAGSP1	CCTGCAAAGCCGCCAGAACTTGAG	60	591, cDNA
		5′NAGSP2	GACTCTGAAGGAACCCCATCTG	60	511, cDNA
	Real-time PCR	QF2	GACTGGAAGAAAGGCAAGAACG	60	128, cDNA
		QR2	GGGCTGAAGAAGTTGAAGAAGG
RPL32	Real-time PCR	QF3	AGCCCAAGATCGTCAAAAAG	60	165, cDNA
		QR3	TGTTGCTCCCATAACCAATG