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Journal of Biomedicine and Biotechnology
Volume 2012 (2012), Article ID 948320, 11 pages
http://dx.doi.org/10.1155/2012/948320
Research Article

Xenogenic Esophagus Scaffolds Fixed with Several Agents: Comparative In Vivo Study of Rejection and Inflammation

1Translational Centre for Regenerative Medicine (TRM), University of Leipzig, 04103 Leipzig, Germany
2Institute of Clinical Immunology, Faculty of Medicine, University of Leipzig, 04103 Leipzig, Germany
3Department of Pediatric Surgery, University of Leipzig, 04103 Leipzig, Germany
4Institute of Pathology, Faculty of Veterinary Medicine, University of Leipzig, 04103 Leipzig, Germany
5Institute of Pathology, University of Leipzig, 04103 Leipzig, Germany

Received 27 September 2011; Revised 14 December 2011; Accepted 15 December 2011

Academic Editor: George E. Plopper

Copyright © 2012 Holger Koch et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Most infants with long-gap esophageal atresia receive an esophageal replacement with tissue from stomach or colon, because the native esophagus is too short for true primary repair. Tissue-engineered esophageal conducts could present an attractive alternative. In this paper, circular decellularized porcine esophageal scaffold tissues were implanted subcutaneously into Sprague-Dawley rats. Depending on scaffold cross-linking with genipin, glutaraldehyde, and carbodiimide (untreated scaffolds : positive control; bovine pericardium : gold standard), the number of infiltrating fibroblasts, lymphocytes, macrophages, giant cells, and capillaries was determined to quantify the host response after 1, 9, and 30 days. Decellularized esophagus scaffolds were shown to maintain native matrix morphology and extracellular matrix composition. Typical inflammatory reactions were observed in all implants; however, the cellular infiltration was reduced in the genipin group. We conclude that genipin is the most efficient and best tolerated cross-linking agent to attenuate inflammation and to improve the integration of esophageal scaffolds into its surrounding tissue after implantation.