950658.fig.002
Figure 2: Salinomycin does not permit long-term adaptation and development of resistance of KG-1a AML SCs to apoptosis-inducing concentrations of salinomycin (Sal, 10 μM), whereas the cells could be readily adapted to survive and to proliferate in the presence of initially apoptosis-inducing concentrations of doxorubicin (Dox, 0.5 μg/mL) and bortezomib (Bor, 12.5 nM). After 12 weeks of culturing in the presence of 12.5 nM Bor, 0.5 μg/mL Dox, 10 μM Sal, or DMSO 5% (v/v), proliferation of the cells was determined by [3H] thymidine incorporation for 24 h. White bars: KG-1a AML SCs; grey bars: KG-1a AML cells; black bars: KG-1 AML cells. Inserts show invert microscopic pictures (400x) of KG-1a AML SCs cultured for 12 weeks in the presence of the drugs noted below. Size bars are 50 μm. Adapted from [16], with permission from Elsevier B.V.