Research Article
Depigmenting Effect of Kojic Acid Esters in Hyperpigmented B16F1 Melanoma Cells
Table 1
The yield of KA esters synthesized by enzymatic esterification using immobilized lipase from Rhizomucor miehei at optimal reaction condition.
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time of enzymatic esterification to achieve maximum concentration of KA esters. Percentage yield was calculated using following equation: Yield (%) = (comp/mole of KA) × dilution factor × 100 comp = (comp/IS) × (IS/RF), where, comp: area for each component; IS: area for internal standard; IS: concentration for internal standard; RF: RF standard/RF internal standard; comp: concentration for each component. |