Role of TGF-β1/Smad3 Signaling Pathway in Secretion of Type I and III Collagen by Vascular Smooth Muscle Cells of Rats Undergoing Balloon Injury
Figure 3
Expressions of type I and III collagen in VSMCs transfected with adenovirus expressing antisense Smad3 in vitro. Total RNA was extracted for RT-PCR. The culture medium was collected, and ELISA was performed to measure the contents of type I and III collagen. (a) mRNA expression of type I and III collagen in three groups; (b) contents of type I and III collagen in three groups. The mRNA expression of type I and III was normalized to that of -actin as the relative expression. Data were expressed as mean ± SD. ▲, ■ versus negative control group.