Role of TGF-β1/Smad3 Signaling Pathway in Secretion of Type I and III Collagen by Vascular Smooth Muscle Cells of Rats Undergoing Balloon Injury
Figure 4
Expressions of type I and III collagen in VSMCs following injection of adenovirus expressing antisense Smad3 in vivo. Ninety SD rats were randomly assigned into blank control group, experiment group, and negative control group. In all groups ( per group), rats were sacrificed at 1 day, 3 days, 1 week, 2 weeks, 1 month, and 3 months after injury followed by sample collection. Total RNA was extracted for the detection of mRNA expressions of type I and III collagen with quantitative PCR (a, c). In addition, the serum was also collected from rats followed by detection of type I and III collagen contents with ELISA (b, d). (a) mRNA expression of type I collagen in different groups; (b) content of type I collagen in the serum of different groups; (c) mRNA expression of type III collagen in different groups; (d) content of type III collagen in the serum of different groups. The mRNA expressions of type I and III collagen were normalized to that of -actin. The mRNA expressions of type I and III collagen in the blank control group served as the baseline levels, and those in other groups were normalized to those in the blank control group as the fold change which was used for delineation. Data were expressed as mean ± SD. ▲, ■ versus negative control group.
