Research Article
Role of TGF-β1/Smad3 Signaling Pathway in Secretion of Type I and III Collagen by Vascular Smooth Muscle Cells of Rats Undergoing Balloon Injury
Table 1
Primers for real-time PCR.
| | Genes | Sequences | Expected size |
| | TGF-β1 | Forward 5′-ACTCCCAGAAAAGCAAGCAA-3′ | 211 bp | | Reverse 5′-CGAGCAGGAATGAGAAGAGG-3′ |
| | Smad3 | Forward 5′-CATTACCATCCCCAGGTCAC-3′ | 102 bp | | Reverse 5′-CGTAACTCATGGTGGCTGTG-3′ |
| | Collagen I | Forward 5′-CCC TGA AGT CAG CTG CAT-3′ | 193 bp | | Reverse 5′-ATA TTC TTC TGG GCA GAA-3′ |
| | Collagen III | Forward 5′-TGGTCCTCAGGGTGTAAAGG-3′ | 232 bp | | Reverse 5′-GTCCAGCATCACCTTTTGG-3′ |
| | β-actin | Forward 5′-CTGTCCCTGTATGCCTCTG-3′ | 217 bp | | Reverse 5′-TGTCACGCACGATTTCC-3′ |
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