Figure 2: (a, b) Photographs of the BAC-DKK3-EGFP mouse embryo at E9.5. The embryo was visualized under bright-field (a) and fluorescence (b). h, heart; sc, spinal cord. (c) A coronal section through the palatal region of the BAC-Dkk3-EGFP mouse at E16.5. t, tooth; gm, genioglossus muscle. (d) A coronal section through the heart of the BAC-DKK3-EGFP mouse at E16.5. PT, pulmonary trunk; Ao, Aorta; LA, left atrium; RA, right atrium; RV, right ventricle. (e–j) EGFP expression in the BAC-Dkk3-EGFP mouse retina. EGFP expression was detected at E15.5 (e), E18.5 (f), P0 (g), P3 (h), P6 (i), and P14 (j). Nuclei were counterstained with DAPI. NBL, neuroblastic layer; ONL, outer nuclear layer; INL, inner nuclear layer. Scale bar represents 50 m.