Research Article

Prevalence of Adhesion and Regulation of Biofilm-Related Genes in Different Clones of Staphylococcus aureus

Table 1

PCR target genes and primers used in this work.

Genes Nucleotide sequence of primersPCR annealing temperature (°C) Amplicon size (bp) Reference

icaA (intercellular adhesion gene) 5-ACACTTGCTGGCGCAGTCAA-3 55°C 188 Rohde et al., 2001 [6]
5-TCTGGAACCAACATCCAACA-3
icaD (intercellular adhesion gene) 5-ATGGTCAAGCCCAGACAGAG-355°C198Rohde et al., 2001 [6]
5-AGTATTTTCAATGTTTAAAGCAA-3
icaB (intercellular adhesion gene) 5-AGAATCGTGAAGTATAGAAAATT-355°C*900Kiem et al., 2004 [7]
5-TCTAATCTTTTTCATGGAATCCGT-3′
icaC (intercellular adhesion gene) 5-ATGGGACGGATTCCATGAAAAAGA-355°C*1100Kiem et al., 2004 [7]
5-TAATAAGCATTAATGTTCAATT-3
eno (laminin binding protein) 5-ACGTGCAGCAGCTGACT-355°C301Tristan et al., 2003 [8]
5-CAACAGCATCTTCAGTACCTTC-3
ebpS (elastin binding protein) 5-CATCCAGAACCAATCGAAGAC-355°C180Tristan et al., 2003 [8]
5- AGTTACATCATCATGTTTATCTTTTG-3
fnbA (fibronectin binding protein A) 5-CATAAATTGGGAGCAGCATCA-355°C*128Vancraeynest et al., 2004 [9]
5- ATCAGCAGCTGAATTCCCATT-3
fnbB (fibronectin binding protein B) 5-GTAACAGCTAATGGTCGAATTGATACT-355°C523Tristan et al., 2003 [8]
5-CAAGTTCGATAGGAGTACTATGTTC-3
fib (fibrinogen binding protein) 5-CTACAACTACAATTGCGTCAACAG-355°C405Tristan et al., 2003 [8]
5-GCTCTTGTAAGACCATTTTCTTCAC-3
clfA (clumping factor A) 5-ATTGGCGTGGCTTCAGTGCT-355°C288Tristan et al., 2003 [8]
5-CGTTTCTTCCGTAGTTGCATTTG-3
clfB (clumping factor B) 5-ACATCAGTAATAGTAGGGGCAAC-355°C203Tristan et al., 2003 [8]
5-TTCGCACTGTTTGTGTTTGCAC-3
bbp (bone sialoprotein binding protein) 5-AACTACATCTAGTACTCAACAACAG-355°C574Tristan et al., 2003 [8]
5-ATGTGCTTGAATAACACCATCATCT-3
cna (collagen binding protein) 5-AAAGCGTTGCCTAGTGGAGA-355°C192Montanaro et al., 1999 [10]
5-AGTGCCTTCCCAAACCTTTT-3

*Optimized PCR annealing temperature program in this study at 55°C.