Research Article
Characterization of Plasminogen Binding to NB4 Promyelocytic Cells Using Monoclonal Antibodies against Receptor-Induced Binding Sites in Cell-Bound Plasminogen
Table 1
Plasminogen binding to cell surfaces of several leukemic cell lines and normal peripheral blood cells.
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Binding analyses were carried out by incubation of washed cells (2–5 × 106/mL) with radiolabeled plasminogen (100 nM) in a total volume of 200 μL for 2 hours a 4°C. Cells were then separate from the whole reaction mixture by centrifugation of aliquots in 20% sucrose solution. The specific binding of radiolabeled plasminogen was determined by subtracting counts bound in the presence of 0.15 M ε-ACA. Results are the mean ± SD of 2–4 separate experiments. |