Table 1: Plasminogen binding to cell surfaces of several leukemic cell lines and normal peripheral blood cells.
Molecules of plasminogen bound/cell ()
Undifferentiated T cell
Binding analyses were carried out by incubation of washed cells (2–5 × 106/mL) with radiolabeled plasminogen (100 nM) in a total volume of 200 μL for 2 hours a 4°C. Cells were then separate from the whole reaction mixture by centrifugation of aliquots in 20% sucrose solution. The specific binding of radiolabeled plasminogen was determined by subtracting counts bound in the presence of 0.15 M ε-ACA. Results are the mean ± SD of 2–4 separate experiments.