Figure 5: Inhibition of CRIM1 increases nuclear accumulation of Flag-NT-PGC-1α. (a) Representative images of Flag-NT-PGC-1α in a control fiber (control) and that of another fiber treated with 40 nM CRM1 inhibitor leptomycin B (LMB) for 20 h in culture medium in the tissue culture incubator. Scale bar, 10 μm. (b) The n/c fluorescence ratio of Flag-NT-PGC-1α in muscle fibers with (LMB) or without (control) leptomycin B treatment. n/c values from 40 nuclei from 40 randomly selected different fibers were averaged to give the mean value for each group. Asterisk indicates statistically significant difference between groups at .