Table 3: The impact of MTX at various concentrations on inflammation-related cytokines in RA and healthy control group.

RANKL 
(pmol/L)
OPG 
(pmol/L)
RANKL/OPGTNF-
(pg/mL)
IL-17 
(pg/mL)
IL-6 
(pg/mL)
IL-4 
(pg/mL)

RA patient
 Anti-CD3/CD28100.72 ± 11.980.57 ± 0.15190.24 ± 51.25508.52 ± 90.94606.76 ± 49.797939.02 ± 2108.859.46 ± 4.15
 M177.60 ± 7.610.44 ± 0.05175.62 ± 21.61318.81 ± 74.45451.50 ± 50.085255.36 ± 4309.0316.30 ± 11.6
 M282.57 ± 11.230.50 ± 0.06164.74 ± 18.24292.46 ± 58.67372.13 ± 66.647251.50 ± 4455.9310.73 ± 6.84
 M377.12 ± 7.360.57 ± 0.13139.14 ± 29.05265.51 ± 64.08315.10 ± 103.734706.41 ± 3391.3414.13 ± 9.24
Healthy control
 Anti-CD3/CD2883.09 ± 12.170.61 ± 0.07136.23 ± 13.42195.95 ± 52.83249.87 ± 17.632607.90 ± 232.986.77 ± 4.43
 M171.02 ± 16.390.45 ± 0.32158.02 ± 38.89161.43 ± 44.09204.60 ± 21.311952.67 ± 355.3515.57 ± 27.02
 M271.41 ± 17.130.53 ± 0.06134.89 ± 34.03144.53 ± 24.13188.03 ± 15.412177.13 ± 315.5516.15 ± 11.55
 M380.49 ± 24.100.57 ± 0.07140.29 ± 42.54128.42 ± 24.88187.83 ± 41.343823.98 ± 2478.597.38 ± 2.82

Values are expressed as mean ± standard deviation.