1,25-Dihydroxyvitamin D3 Inhibits the RANKL Pathway and Impacts on the Production of Pathway-Associated Cytokines in Early Rheumatoid Arthritis

Table 3

The impact of MTX at various concentrations on inflammation-related cytokines in RA and healthy control group.

	RANKL; (pmol/L)	OPG; (pmol/L)	RANKL/OPG	TNF-𝛼; (pg/mL)	IL-17; (pg/mL)	IL-6; (pg/mL)	IL-4; (pg/mL)
RA patient
Anti-CD3/CD28	100.72 ± 11.98	0.57 ± 0.15	190.24 ± 51.25	508.52 ± 90.94	606.76 ± 49.79	7939.02 ± 2108.85	9.46 ± 4.15
M1	77.60 ± 7.61	0.44 ± 0.05	175.62 ± 21.61	318.81 ± 74.45	451.50 ± 50.08	5255.36 ± 4309.03	16.30 ± 11.6
M2	82.57 ± 11.23	0.50 ± 0.06	164.74 ± 18.24	292.46 ± 58.67	372.13 ± 66.64	7251.50 ± 4455.93	10.73 ± 6.84
M3	77.12 ± 7.36	0.57 ± 0.13	139.14 ± 29.05	265.51 ± 64.08	315.10 ± 103.73	4706.41 ± 3391.34	14.13 ± 9.24
Healthy control
Anti-CD3/CD28	83.09 ± 12.17	0.61 ± 0.07	136.23 ± 13.42	195.95 ± 52.83	249.87 ± 17.63	2607.90 ± 232.98	6.77 ± 4.43
M1	71.02 ± 16.39	0.45 ± 0.32	158.02 ± 38.89	161.43 ± 44.09	204.60 ± 21.31	1952.67 ± 355.35	15.57 ± 27.02
M2	71.41 ± 17.13	0.53 ± 0.06	134.89 ± 34.03	144.53 ± 24.13	188.03 ± 15.41	2177.13 ± 315.55	16.15 ± 11.55
M3	80.49 ± 24.10	0.57 ± 0.07	140.29 ± 42.54	128.42 ± 24.88	187.83 ± 41.34	3823.98 ± 2478.59	7.38 ± 2.82

Values are expressed as mean ± standard deviation.