102918.fig.005a
(a)
102918.fig.005b
(b)
102918.fig.005c
(c)
102918.fig.005d
(d)
Figure 5: Effect of GSC extract on the level of TNF-α and iNOS mRNA expression and NO production in LPS-stimulated RAW264.7 macrophages. ((a), (b)) Levels of TNF-α and iNOS mRNA were determined by quantitative Real-Time-PCR, using GAPDH mRNA as the internal control. The graph shown is representative of three independent experiments ( , versus control; , versus DSS). (c) NO production in LPS-stimulated RAW264.7 cells. (d) RAW264.7 cell viability. One-way ANOVA was used for comparisons of multiple group means, followed by Dunnett’s -test ( versus control; , versus LPS). LPS: lipopolysaccharide. The graph shown is representative of three independent experiments.