Extending the Serum Half-Life of G-CSF via Fusion with the Domain III of Human Serum Albumin

Figure 2

Analysis of transformants that expressed 3DHSA-G-CSF by SDS-PAGE. The culture supernatants of screened transformant were resolved by 12% SDS-PAGE and stained with Coomassie Blue. M: protein marker; Lanes 1–8 correspond to different transformants of GS115-pPIC-3DHSA-G-CSF; the protein band about 42 kDa was marked 3DHSA-G-CSF on the right of SDS-PAGE.