Research Article
Immune Parameters in The Prognosis and Therapy Monitoring of Cutaneous Melanoma Patients: Experience, Role, and Limitations
Table 1
Immunohistochemical markers.
| | Antibody | Source | Clone | Host | Working dilution | Pretreatment* |
| | CD3 | Leica/Novocastra | PS1 | Mouse | 0.3/200 | HIER, buffer citrate, and pH 6 | | CD5 | idem | 4C7 | idem | 1/200 | idem | | CD4 | idem | 4B12 | idem | 5/200 | idem | | CD23 | idem | 1B12 | idem | RTU | idem | | Langerin | idem | 12D6 | idem | 0.5/200 | idem | | CD1a | idem | JPM30 | idem | 2/160 | idem | | CD7 | Thermo Scientific/Neomarkers | 272 | idem | RTU | idem | | CD8 | idem | C8/144B | idem | 2/200 | idem | | CD20 | Cell Marque | L26 | | RTU | idem | | CD138 | Invitrogen | — | Rabbit | 1/200 | HIER, EDTA citrate, and pH 8 |
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