Research Article

The Glycosylation of AGP and Its Associations with the Binding to Methadone

Table 2

Intrinsic fluorescence of AGP in absence and presence of 1.07 μM and 250 μM methadone.

Sample Fluorescence in the absence of methadone (RFU) Fluorescence in the presence of 1.07  M methadone (RFU) Fluorescence in the presence of 250  M methadone (RFU)

Commercial (sigma) 41636 38236 30620
“Normal” 1 39943.5 39021 35476
“Normal” 2 37784.5 37032 36138
T1 39859 34006 30712
T2 35992 33371 30840
T3 36715 33020 30284
T4 37571 31089 29182
T5 38882 33959 29412
HR1 39107 33280 29289
HR2 38438 33215 29025
HR3 33531 30023 27072
LT1 31770 30775 27196
LT2 33324 30252 28346
LT4 38688 37189 31008
LT5 39937 36241 31893
LT6 39691 31681 27137
LT7 38484 31538 28658