IL-6 Production by Dendritic Cells Is Dispensable for CD8⁺ Memory T-Cell Generation

Figure 6

Generation of functional OVA-specific CD8⁺ memory T cells following immunization with IL-6-deficient DCs. (a) Functionality of OVA-specific CD8⁺ Tm cells at day 60 post-immunization. The overlays show production of the different effector molecules by OVA-specific T cells (CD8⁺CD45.2⁺) compared to endogenous T cells (CD8⁺CD45.2⁻) following immunization with WT (top) or IL-6 KO (bottom) DCs. The percentage of cells producing the different effector molecules is indicated on each histogram. (b) Quantification of cytokine and granzyme B production by OVA-specific CD8⁺ Tm cells. The percentage of cytokines and granzyme B producing OVA-specific Tm cells (top) and the amount produced (bottom) are shown at day 60 post-immunization. The MFI of cytokine and granzyme B production by CD8⁺ Tm cells was normalized to the MFI of the recipient CD8⁺ T cells (MFI ratio). The results are from two independent experiments.

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