126189.fig.006a
(a)
126189.fig.006b
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Figure 6: Generation of functional OVA-specific CD8+ memory T cells following immunization with IL-6-deficient DCs. (a) Functionality of OVA-specific CD8+ Tm cells at day 60 post-immunization. The overlays show production of the different effector molecules by OVA-specific T cells (CD8+CD45.2+) compared to endogenous T cells (CD8+CD45.2) following immunization with WT (top) or IL-6 KO (bottom) DCs. The percentage of cells producing the different effector molecules is indicated on each histogram. (b) Quantification of cytokine and granzyme B production by OVA-specific CD8+ Tm cells. The percentage of cytokines and granzyme B producing OVA-specific Tm cells (top) and the amount produced (bottom) are shown at day 60 post-immunization. The MFI of cytokine and granzyme B production by CD8+ Tm cells was normalized to the MFI of the recipient CD8+ T cells (MFI ratio). The results are from two independent experiments.