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BioMed Research International
Volume 2013 (2013), Article ID 126923, 7 pages
http://dx.doi.org/10.1155/2013/126923
Research Article

Preparation, Characterization, and Determination of Immunological Activities of Transfer Factor Specific to Human Sperm Antigen

1Clinical Laboratory, The Affiliated Hospital of Jining Medical College, Shandong, Jining 272029, China
2Department of Immunology, Zunyi Medical College, Guizhou, Zunyi 563003, China
3Medical Reproduction Centre, The Affiliated Hospital of Jining Medical College, Shandong, Jining 272029, China

Received 16 August 2012; Revised 22 October 2012; Accepted 7 November 2012

Academic Editor: Andre Van Wijnen

Copyright © 2013 Jianwei Zhou et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective. The objective of this study was to prepare, characterize, and determine immunological activities of specific transfer factor (STF) specific to human sperm antigen (HSA) for the preparation of antisperm contraceptive vaccine that can be used as an immunocontraceptive. Methods. HSA-STF was prepared using the spleens of rabbits vaccinated with HSA. The specific immunological activities were examined by lymphocyte proliferation test (LPT), leukocyte adhesion inhibition test (LAIT), and by determining the concentrations of IL-4, γ-IFN, and IL-21. HSA-STF was a helveolous substance, having a pH value of and UV absorption maxima at  nm. It contained seventeen amino acids; glycine and glutamic acids were the highest in terms of concentrations (38.8 μg/mL and 36.3 μg/mL, resp.). Results. The concentration of polypeptide was  mg/mL, and ribose was  mg/mL. The stimulation index for lymphocyte proliferation test was 1.84, and the leukocyte adhesion inhibition rate was 37.7%. There was a statistically significant difference between the cultural lymphocytes with HSA-STF and non-HSA-STF for γ-IFN and IL-21 ( ), but there was no statistical significance for IL-4 ( ). Conclusion. HSA-STF was prepared and characterized successfully. It had immunological activity which could transfer the immune response specific to HSA and prove to be a potential candidate for the development of male immunocontraceptive agents.