Interferon-α Enhances 5′-Deoxy-5-Fluorouridine-Induced Apoptosis by ERK-Dependant Upregulation of Thymidine Phosphorylase
Figure 2
IFN-α upregulated the expression of TP in gastric cancer cells. (a) SGC7901 and MGC803 cells were treated with 1000 IU/mL of IFN-α for 24 h and 48 h. The expression of TP proteins was analyzed by Western blot. Data were means ± SD of three independent experiments. *Incubated with IFN-α for 24 h or 48 h versus that with IFN-α for 0 h, respectively, . (b) SGC7901 and MGC803 cells were preincubated with 1000 IU/mL of IFN-α for 48 h, followed by treatment with 250 μg/mL of -DFUR for 48 h. The expression of TP proteins was analyzed by Western blot. Data were means ± SD of three independent experiments. *Incubated with IFN-α alone or with IFN-α and -DFUR versus that without both, respectively, . (c) MGC803 cells were transiently transfected with TP siRNA for 48 h, followed by 1000 IU/mL of IFN-α or 1000 IU/mL of IFN-α and 250 μg/mL of -DFUR. TP expression was analyzed by Western blot. Data were means SD of three independent experiments. *Incubated with IFN-α alone or with IFN-α and -DFUR or without both in siRNA TP versus that in siRNA control, respectively, . (d) SGC7901 and MGC803 cells were transiently transfected with TP siRNA for 48 h, followed by 1000 IU/mL of IFN-α or 1000 IU/mL of IFN-α and 250 μg/mL of -DFUR. Cell apoptosis was quantified by flow cytometry. Data were means ± SD of three independent experiments. *Incubated with IFN-α and -DFUR in siRNA TP versus that in siRNA control, .
