Research Article
Development of a Novel Reference Plasmid for Accurate Quantification of Genetically Modified Kefeng6 Rice DNA in Food and Feed Samples
Table 1
Primers and probes used in this study.
| Target | Purpose | Primer sequence (5′→3′) | Amplicon size |
| Kefeng6 | Construction | KF6-1F GGATCCACGTAGTACGTACCGCCGTG1 | 89 |
KF6-1R GGATCCAGTGCAGATGCATGAATCGC1 | Quantification | KF6-2F ACGTAGTACGTACCGCCGTG | 77 | KF6-2R AGTGCAGATGCATGAATCGC | KF6-P FAM-CCGCGCGTTGTACTGAGAACCA-TAMRA |
| gos9 | Construction | gos9-1F GGATCCTTAGCCTCCCGCTGCAGA1 | 80 |
gos9-1R AAGCTTAGAGTCCACAAGTGCTCCCG2 | Quantification | gos9-2F TTAGCCTCCCGCTGCAGA | 68 | gos9-2R AGAGTCCACAAGTGCTCCCG | gos9-P FAM-CGGCAGTGTGGTTGGTTTCTTCGG-TAMRA |
| Kefeng6 and gos9 | Fusion | Fusion-F GGGAGGCTAAGGATCCAGTGCAGATG | 183 | Fusion-R CATCTGCACTGGATCCTTAGCCTCCC |
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BamHI site in bold type, 2HindIII site in bold type.
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