Research Article
Development of a Novel Reference Plasmid for Accurate Quantification of Genetically Modified Kefeng6 Rice DNA in Food and Feed Samples
Table 2
Optimized concentrations for qPCR and dPCR assays.
| | Component | qPCR | dPCR | | Kefeng6 (L) | gos9 (L) | Kefeng6 (L) | gos9 (L) |
| | TaqMan Universal Master Mix (2) | 12.5 | 12.5 | 5 | 5 | | Primer-F (10 M) | 1 | 1 | 0.4 | 0.4 | | Primer-R (10 M) | 1 | 1 | 0.4 | 0.4 | | Probe-P (10 M) | 0.5 | 0.5 | 0.2 | 0.2 | | Template (20 ng/L) | 5 | 5 | 2 | 2 | | Loading (10) | 0 | 0 | 1 | 1 | | H2O | 5 | 5 | 1 | 1 |
| | Total | 25 | 25 | 10 | 10 |
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