Development of a Novel Reference Plasmid for Accurate Quantification of Genetically Modified Kefeng6 Rice DNA in Food and Feed Samples

Table 3

Copy number concentrations and ratios of the third sample (S3) as determined by dPCR.

Kefeng6				gos9				Concentration		Ratio	𝑃
Positive partitions	Estimated copies	Average Number	RSD (%)	Positive partitions	Estimated copies	Average number	RSD (%)	Mean (cp/μL)	RSD (%)	KF6/gos9
494	786	794	2.6	476	774	790	3.3	792	2.87	1.005	0.786
471	780			494	821
516	823			492	784
465	797			455	769
481	811			446	767
455	766			488	825

The average number was calculated from six replicate measurements with three parallel panels per chip (𝑛=6), and this value was adjusted to account for the gravimetrically prepared PCR solutions used in the Kefeng6 and gos9 assays. RSD: relative standard deviation; 𝑃: probability.