An Active C-Terminally Truncated Form of Ca2+/Calmodulin-Dependent Protein Kinase Phosphatase-N (CaMKP-N/PPM1E)
Figure 2
hCaMKP-N and its truncation mutant prepared by a wheat-embryo cell-free protein expression system. (a) hCaMKP-N(WT) (lane 1, 2.5 μg) and hCaMKP-N(1–559) (lane 2, 2.5 μg), which had been prepared and purified as described in Section 2, were subjected to SDS-PAGE on a 10% polyacrylamide gel. The gel was stained with Coomassie Brilliant Blue (CBB) using Quick CBB. (b) hCaMKP-N(WT) (lane 1) and hCaMKP-N(1–559) (lane 2) were subjected to SDS-PAGE on a 10% polyacrylamide gel and analyzed by western blotting analysis using an anti-CaMKP-N antibody (1 : 250 dilution) as the primary antibody.
