An Active C-Terminally Truncated Form of Ca²⁺/Calmodulin-Dependent Protein Kinase Phosphatase-N (CaMKP-N/PPM1E)

Table 1

Comparison of the phosphatase activities of hCaMKP-N(WT) and hCaMKP-N(1–559). hCaMKP-N(WT) and hCaMKP-N(1–559) were assayed using varying concentrations of the pp10 substrate as described in Section 2. Michaelis-Menten kinetic parameters were determined from a direct fit to the Michaelis-Menten equation. The data represent the average of three independent experiments ± S.D.

	𝐾𝑚 (μM)	𝑉max (nmol/min/mg)
hCaMKP-N(WT)	2.0±0.9	1.4±0.1
hCaMKP-N(1–559)	5.4±0.1	16.3±3.6