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BioMed Research International
Volume 2013 (2013), Article ID 135086, 13 pages
A Pipeline with Multiplex Reverse Transcription Polymerase Chain Reaction and Microarray for Screening of Chromosomal Translocations in Leukemia
1School of Life Science and Technology, Tongji University, Shanghai 200092, China
2National Engineering Research Center for Biochip at Shanghai, Shanghai 201203, China
3Department of Hematology and Oncology, Shanghai Children's Medical Center, Shanghai Jiaotong University School of Medicine, Shanghai 200127, China
4State Key Laboratory of Medical Genomics and Shanghai Institute of Hematology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China
5Wayen Biotechnologies Inc., Shanghai 201203, China
Received 21 May 2013; Revised 1 August 2013; Accepted 19 August 2013
Academic Editor: Luca Arcaini
Copyright © 2013 Fei-Fei Xiong et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Supplementary Text 1: Methods for clinic diagnosis of 200 leukemia samples, including cytogenetic, FISH and RT-PCR analysis.
Supplementary Figure 1: Results from the multiplex RT-PCR combined with microarray for positive controls.
Supplementary Table 1: Multiplex RT-PCR - microarray and clinic diagnosis of 200 leukemia samples.